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. 2016 Apr 5;291(21):11161–11171. doi: 10.1074/jbc.M116.724849

FIGURE 5.

FIGURE 5.

ARL3 is involved in phosphorylation of STAT3. A, HeLa cells in a 24-well plate were transfected with control, huARL3#1, or huARL3#2 siRNAs (20 pmol). 48 h after transfection, the cells were treated with IL-6 (10 ng/ml) for the indicated periods. Then the cells were lysed, and an aliquot of total cell lysates (1%) was blotted with anti-pSTAT3 (Tyr-705), anti-pSTAT3 (Ser-727), anti-STAT3, or anti-ARL3 antibody. IB, immunoblot. B, densitometric quantification of the above results was also shown. Relative intensity of pSTAT3 (Tyr-705) was normalized to the STAT3 protein of the same sample. The results are representative of three independent experiments, and the error bars represent the S.D. **, p < 0.01. C, HeLa shControl, shARL3#2-1, or shARL3#2-10 cells in a 24-well plate were treated with IL-6 (10 ng/ml) for the indicated periods. Then the cells were lysed, and an aliquot of total cell lysates (1%) was blotted with anti-pSTAT3 (Tyr-705), anti-pSTAT3 (Ser-727), anti-STAT3, or anti-ARL3 antibody. D, densitometric quantification of the above results was also shown. Relative intensity of pSTAT3 (Tyr-705) was normalized to the STAT3 protein of the same sample. The results are representative of three independent experiments, and the error bars represent the S.D.