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. 2016 Mar 18;291(21):11268–11284. doi: 10.1074/jbc.M115.703868

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Protein production quantification from Western blotting analysis for FGF14^Y158A, FGF14^V160A, and FGF14^Y158A/V160A. A, Western blotting of whole-cell extracts from cells transfected with the indicated CLuc-FGF14 and CD4-Nav1.6-NLuc constructs. B, summary graph of densitometry analysis of CLuc and NLuc band intensity ratio of the respective protein products. C, Western blotting of whole-cell extracts from cells transfected with the indicated CLuc-FGF14 and FGF14-NLuc constructs. D, summary graph of densitometry analysis as described in C. Membrane were probed with anti-luciferase antibodies that recognize either the CLuc or the NLuc fragments (∼46 and ∼66/114 kDa, respectively). Immunodetection of calnexin was used as loading control.