TABLE 5.
Nav1.6-mediated currents in the presence of FGF14 and Lys-74 and Ile-76 mutants
The number of independent experiments is shown in parentheses.
| Condition | Peak density | Activation | Kact | Inactivation | Kinact | τ |
|---|---|---|---|---|---|---|
| pA/pF | mV | mV | mV | mV | ms | |
| GFP | −43.2 ± 5.1 (21) | −20.8 ± 0.9 (19) | 4.7 ± 0.2 (19) | −62 ± 1.4 (15) | 6.3 ± 0.3 (15) | 1.0 ± 0.08 (14) |
| FGF14-GFP | −9.3 ± 1.8 (12)a | −15.7 ± 0.9 (11)b | 6.2 ± 0.4 (11)c | −56.0 ± 1.0 (10)c | 7.1 ± 1.1 (10) | 1.7 ± 0.18 (10)d |
| FGF14K74A-GFP | −21.2 ± 2.5 (8) | −19.4 ± 1.1 (8) | 4.8 ± 0.2 (8) | −71.9 ± 5.9 (8) | 11.2 ± 1. 1 (8) | 1.9 ± 0.5 (8)d |
| FGF14I76A-GFP | −10.9 ± 2.4 (7)e | −18.6 ± 1.2 (7) | 6.2 ± 0.3 (7)c | −86.6 ± 3.5 (7)f | 15.7 ± 1.0 (7)g | 1.7 ± 0.2 (7)d |
| FGF14K74A/I76A-GFP | −46.8 ± 13.6 (10) | −27.4 ± 2.9 (8) | 3.9 ± 0.7 (8) | −62.5 ± 3.0 (9) | 8.8 ± 1.2 (9) | 2.0 ± 0.2 (9)d |
a p < 0.001, one-way ANOVA, post hoc Dunn's multiple comparisons test compared with GFP; data are mean ± S.E.
b p < 0.01, one-way ANOVA, post hoc Dunn's multiple comparisons test compared with GFP; data are mean ± S.E.
c p < 0.05, one-way ANOVA, post hoc Dunnett's multiple comparisons test compared with GFP; data are mean ± S.E.
d p < 0.05, one-way ANOVA, post hoc Dunn's multiple comparisons test compared with GFP; data are mean ± S.E.
e p < 0.01, one-way ANOVA, post hoc Dunn's multiple comparisons test compared with GFP; data are mean ± S.E.
f p < 0.05, student-t test compared with GFP; data are mean ± S.E.
g p < 0.01, one-way ANOVA, post hoc Dunn's multiple comparisons test compared with GFP; data are mean ± S.E.