FIGURE 2.

PAR₂-induced activation of PKD. Western blots for Ser⁹¹⁶ phospho-PKD (p-PKD) and total PKD in HEK293 cell are shown. Cells were unstimulated (control, Con.) or were stimulated with trypsin (Tryp., 10 nm) or 2-furoyl-LIGRLO-NH₂ (2F, 10 μm) for 5 min. Cells were preincubated with CRT0066101 (CRT) or vehicle (Veh.). A and B, representative blots. C and D, quantified results. *, p < 0.05, n = 3 experiments.