FIGURE 1.

Analysis of seed proteins of M. pruriens by 12% SDS-PAGE in reducing conditions after ammonium sulfate fractionation. A, lanes 2–7, 0–30%, 30–40%, 40–50%, 50–60%, 60–70%, and 70–80% fractions, respectively. In lane 1, low molecular weight protein markers (Sigma-Aldrich) were loaded. B, 40% fraction highlights two major protein bands, MP-1 and MP-6; the 60% fraction shows four major protein bands, MP-2, MP-3, MP-4, and MP-5. C, N-terminal sequences by Edman degradation method of all of the major proteins present in 40 and 60% ammonium sulfate fractions.