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. 2016 Mar 17;291(21):11373–11384. doi: 10.1074/jbc.M115.699173

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Binding analysis of MP-4 with trypsin and chymotrypsin by SPR. A, SPR sensorgram for the binding of MP-4 (ligand) to trypsin (analyte) immobilized on the surface of a CM5 chip. The ligand was tested in the concentration range from 16 to 0.125 μm. B, binding of MP-4 (ligand) to chymotrypsin (analyte), where chymotrypsin was used in the range from 32 to 0.125 μm. Shown are the equilibrium rate constant, R_max, and χ² value for MP-4·trypsin and MP-4·chymotrypsin complex over the corresponding sensorgram. RU, response units.