Table 1.
Identification of Root Microsomal Proteins in Arabidopsis Using MALDI-TOF MS
| Spot No.a | Apparent MM (kD)/pIb | Match MM (kD)/pIc | MOWSE Scored | No. MPe | Percent Coveredf | Accession No.g | Protein Nameh |
|---|---|---|---|---|---|---|---|
| 3 | 73.04/6.4 | 59.72/6.4 | 2.77E+09 | 20 | 43 | 15232626 | Thioglucosidase 3D precursor |
| 4 | 71.07/6.5 | 59.75/6.4 | 6.96E+04 | 13 | 30 | 1363489 | Thioglucosidase 3D precursor |
| 7 | 62.99/5.4 | 63.37/6.5 | 5407 | 7 | 19 | 1732570 | Mitochondrial F1 ATP synthase β subunit |
| 8 | 62.99/5.5 | 63.37/6.5 | 1.87E+13 | 24 | 47 | 1732570 | Mitochondrial F1 ATP synthase β subunit |
| 9 | 62.99/5.6 | 63.37/6.5 | 1.68E+08 | 14 | 31 | 1732570 | Mitochondrial F1 ATP synthase β subunit |
| 17 | 37.24/5.2 | 24.54/4.9 | 6058 | 7 | 34 | 21554133 | Endomembrane-associated protein |
| 19 | 34.64/5.0 | 41.86/5.4 | 7.87E+04 | 14 | 29 | 15222075 | Actin 8 |
| 20 | 34.53/5.1 | 32.02/5.1 | 4288 | 8 | 29 | 15228216 | Putative lectin |
| 21 | 33.63/5.1 | 29.16/5.1 | 6.62E+04 | 7 | 35 | 21595512 | Caffeoyl-CoA O-methyltransferase-like |
| 23 | 34.87/5.4 | 32.16/5.5 | 8624 | 8 | 36 | 15228198 | Putative lectin |
| 24 | 34.76/5.5 | 32.12/5.5 | 5.57E+05 | 11 | 66 | 21594017 | Putative lectin |
| 30 | 26.45/5.3 | 28.17/5.7 | 7.24E+04 | 12 | 47 | 21536745 | Ferritin 1 precursor |
| 33 | 40.42/5.2 | 35.78/5.2 | 1.47E+06 | 11 | 44 | 1429207 | Annexin |
| 34 | 40.42/5.3 | 35.78/5.2 | 3.17E+10 | 19 | 68 | 1429207 | Annexin |
| 38 | 25.74/6.0 | 21.80/6.0 | 6.41E+06 | 7 | 47 | 15239652 | 1,4-Benzoquinone reductase-like; Trp repressor binding protein-like |
| 57 | 61.29/5.0 | 54.74/5.0 | 7.51E+06 | 14 | 29 | 137465 | Vacuolar ATP synthase subunit B |
| 58 | 61.29/5.0 | 54.74/5.0 | 9.82E+07 | 15 | 34 | 137465 | Vacuolar ATP synthase subunit B |
| 59 | 61.29/5.1 | 54.74/5.0 | 3.47E+04 | 9 | 18 | 137465 | Vacuolar ATP synthase subunit B |
| 61 | 18.56/5.0 | 15.08/5.1 | 1.89E+06 | 9 | 63 | 15238776 | Cytochrome b5 |
| 81 | 48.37/5.4 | 42.62/5.4 | 5.68E+06 | 12 | 33 | 18391442 | Vacuolar ATP synthase subunit C, putative |
| 86 | 70.30/5.3 | 55.33/5.2 | 9.36E+04 | 13 | 34 | 6685244 | ATP synthase α chain |
| 96 | 40.16/5.7 | 35.32/5.6 | 8.98E+04 | 9 | 36 | 1754983 | Strictosidine synthase |
| 97 | 38.86/5.7 | 35.32/5.6 | 1436 | 5 | 22 | 1754983 | Strictosidine synthase |
Number of spot.
Observed molecular mass (MM) and pI of spot from the gel.
Predicted molecular mass (MM) and pI of matched sequence.
Molecular weight search score.
Number of peptides matching to predicted protein sequence.
Percentage of predicted protein sequence covered by matched peptides.
Accession number against the National Center for Biotechnology Information (NCBI) nonredundant database.
Entry name according to the NCBI database.