Figure 3.

Ectopic AG Expression Mediates tcf but Not Phyllotaxy Defects of blr-4.

All plants in this figure were grown at 29°C. Numbers indicate the stage of flowers. The AG:GUS reporter expression ([A] to [C]) is indicated by blue (strong) to pink (weak) staining.

(A) A longitudinal section of a blr-4 shoot before exhibiting tcf. The AG:GUS reporter expression reflects normal AG mRNA expression pattern in whorls 3 and 4 of flowers at stage 4 and stage 6.

(B) A blr-4 shoot at the beginning tcf phase. Ectopic AG expression is detected in all floral whorls, in SAM, and in the stem.

(C) A blr-4 shoot at the later phase of tcf. SAM (arrow) becomes flattened and difficult to identify. Ectopic AG is seen throughout the FM, SAM, and the stem.

(D) An ag-1 flower.

(E) An ag-1 blr-4 double mutant flower resembling ag-1 flowers.

(F) A blr-4 plant showing the abnormal phyllotaxy. A cluster of siliques (arrow) originates from the same position on a stem.

(G) An ag-1 plant with normal phyllotaxy.

(H) A blr-4 ag-1 plant showing clusters of flowers (arrow).