Table 1.

Alterations of Circadian Period in SPY Loss-of-Function and Gain-of-Function Seedlings

Condition	Genotype	Period	RAEa	Amplitude	N
Light entrainment	Col	24.52 ± 0.14	0.12 ± 0.006	1.26 ± 0.12	12
	spy-3	25.38b ± 0.27	0.22 ± 0.023	1.45 ± 0.12	12
	Ler	24.18 ± 0.16	0.16 ± 0.010	1.25 ± 0.09	9
	spy-5	25.19b ± 0.15	0.19 ± 0.020	1.25 ± 0.18	9
	Col	24.54 ± 0.12	0.22 ± 0.015	0.97 ± 0.24	4
	spy-4	26.21b ± 0.31	0.28 ± 0.034	1.37 ± 0.35	7
	35S-SPY	22.91b ± 0.24	0.24 ± 0.012	2.3b ± 0.32	11
	gi-2c	24.26	0.08	0.60
	spy-4 gi-2c	27.42	0.35	0.54
	gi-1c	22.29	0.12	0.90
	gi-3c	21.24	0.25	0.45
	gi-4c	19.44	0.09	0.38
	gi-5c	19.53	0.18	0.63
	gi-6c	18.66	0.17	0.43
Temperature entrainment	Col	24.34 ± 0.15	0.27 ± 0.036	1.01 ± 0.18	15
	spy-4	26.53b ± 0.48	0.27 ± 0.028	1.21 ± 0.12	10
	35S-SPY	22.70b ± 0.39	0.19 ± 0.010	2.29b ± 0.52	4
	gi-2c	25.32	0.14	0.55
	gi-2 spy-4c	26.95	0.20	0.55

Seedlings were grown under entraining conditions for 5 to 7 d before being transferred to 24-well cloning plates in constant light (30 to 40 μmol s⁻¹ m⁻²) and temperature (22°C) for data collection. All numbers are provided ± se.

^aRelative amplitude error.

^bStatistically different from the respective wild type by Student's t test (P < 0.01).

^cThe low amplitude of these genotypes did not allow for an accurate period estimate; therefore, the circadian parameters of the average trace are given as an indication.