Figure 2.

GCR1 Interacts with GPA1 in Planta.

Soluble (S) and membrane (M) protein fractions isolated from gcr1-3 and GCR1:FLAG (Dex-induced [lane 3] and noninduced [lane 4]) plants were used for in vivo coimmunoprecipitation. Proteins immunoprecipitated with anti-GPA1 antibodies were probed with anti-FLAG antibodies by immunoblotting and vice versa. Anti-GPA1 antibodies could pull down GCR1 (closed arrowhead, lanes 1S and 1M) and anti-FLAG antibodies could pull down GPA1 (open arrowhead, lanes 3S and 3M). No signal was detected in gcr1-3 knockout plants (lanes 2S and 2M) or noninduced GCR1:FLAG plants (lanes 4S and 4M).