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. 2004 Jun;16(6):1616–1632. doi: 10.1105/tpc.020321

Figure 3.

Figure 3.

Topology Prediction of GCR1 Fragments Fused with Ubiquitin at the N or C Terminus as Used for Split Ubiquitin Assays.

Schematic representation of topology of GCR1 full-length and truncated proteins with or without a ubiquitin (N-terminal part, Nub) fusion. Topology was predicted using transmembrane domain hidden Markov model (TMHMM version 2.0). Rectangles represent plasma membrane. Predicted transmembrane regions are numbered from 1 to 7. Ub represents N-terminal portion of ubiquitin.

(A) GCR1 full-length protein (GCR1FL) without or with Ub fusion at the N terminus (Nub-GCR1FL) or at the C terminus (GCR1FL-Nub).

(B) GCR1IL2 protein (amino acids 105 to 326) without or with Ub fusion at the N terminus (Nub-GCR1IL2) or at the C terminus (GCR1IL2-Nub).

(C) GCR1IL3 protein (amino acids 271 to 326) without or with Ub fusion at the N terminus (Nub-GCR1IL3) or at the C terminus (GCR1IL3-Nub).