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. 2004 Jun;16(6):1616–1632. doi: 10.1105/tpc.020321

Figure 6.

Figure 6.

GCR1 and GPA1 Both Associate with the Membrane Fraction.

(A) GCR1 is mostly associated with the membrane fraction. Total protein was isolated from GCR1:FLAG plants after 24 h of Dex induction (1) or from gcr1-3 mutant plants (2). Proteins were fractionated into soluble (S) or membrane (M) fractions. Equal amounts of protein were separated on SDS-PAGE and subjected to immunoblotting using anti-FLAG antibodies.

(B) gcr1 mutant plants have lower levels of GPA1 protein. Total proteins were isolated from Ws, Col, gcr1-3, and gcr1-4 leaves and fractionated into soluble and membrane fractions. Equal protein amounts (20 μg/lane) were separated on SDS-PAGE and probed with anti-GPA1 antibodies. GPA1 could be detected only in the membrane fraction. Both of the mutant lines consistently show lower levels of GPA1 protein (gcr1-3, lane 2; gcr1-4, lane 4) compared with the wild-type plants (Ws, lane 1; Col, lane 3).