Fig. 4.

Immunofluorescence staining of TREM-1, CD86+ M1-macrophages and CD106+ M2-macrophages in tissue sections of asymptomatic (AS) and symptomatic (S) carotid plaques. Representative images are shown for TREM-1 (red) and CD86 (green) expression as visualized by dual immunofluorescence in the tissue sections of asymptomatic (AS: Panels, A-D) and symptomatic (S: Panels, E-F). Co-localization of TREM-1 (red) and CD206 (green) is shown for AS (Panels I-L) and S (Panels M-P). Density of CD86⁺ M1- and CD206⁺ M2-macrophages was counted per mm² from 5 different tissues in each experimental group and the data are shown in the bar graph (lower panel). Data are presented as mean±SD; N=5. *p<0.05, ***p<0.001, ****p<0.0001. Scale bar=200 µm for all images.