Fig 7. The point mutation Y265D abolishes Cx43/Drb1-300 binding.
(A) SDS-PAGE gel stained by SR after co-IP (representative lanes chosen as best example from four independent experiments), where GST-Cx43-CT Y265D was used as a “bait” and Drb1-300 as “prey”. Arrows indicate the migration bands of protein A (black), the heavy chain of the IF1 antibody (blue), GST-Cx43-CT (purple), and Drb1-300 (green). Co-IP obtained with the GST-Cx43-CT WT is represented for comparison. (B) Quantification of the data shown in (A). Drb1-300 co-IP band intensities were normalized to the intensity of the protein A band. P value obtained through T-test are indicated. Error bars represent the standard deviation between four replicates.