Fig 1. Image illustrating immunofluorescent cytology approach to measuring the genome-wide recombination rate (from Balcova et al., 2016).

Pachytene spread of a spermatocyte from the C57/BL6 strain shows central elements of synaptonemal complexes of 19 autosomes and the pseudoautosomal region (PAR) immunostained for SYCP1 (green), MLH1 foci (red), and foci of centromeric proteins (violet). The number of MLH1 foci is used as an equivalent of the number of crossovers.