Table 7. Comparison of performance of five approaches for generating our mitogenome sequence data from eight samples of Seychelles caecilians.
Method | Sequencing | Sample preparation time | Sample preparation cost | Sequencing running time | Sequencing cost | Mitogenome reconstruction time | Total time expenditure |
---|---|---|---|---|---|---|---|
Traditional | Sanger | *** | ** | * | * | *** | *** |
Shotgun | Illumina MiSeq | * | * | ** | ** | * | * |
Multiplex | Illumina HiSeq | *** | ** | *** | *** | *** | ** |
Multiplex | Roche 454 | *** | ** | ** | ** | ** | ** |
Multiplex | Ion Torrent | *** | ** | * | * | ** | ** |