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. 2016 Jun 9;11(6):e0157043. doi: 10.1371/journal.pone.0157043

Fig 4. NaCl effect on SPI-1 induction and invasiveness.

Fig 4

A, B. PprgH activity in bacteria grown in LB (A) or saccharate M9 (B) supplemented with the indicated amounts of NaCl, representative of three independent experiments. C, D. Peak promoter activities normalized to 5 g/L LB (C) or 0.5 g/L NaCl saccharate M9 (D). E, F. Flow cytometry from a single representative experiment showing GFP-intensity for the PprgH reporter strain grown in LB (E) or saccharate M9 (F) supplemented with the indicated concentrations of NaCl. The insets show MFI of gated GFP+ bacteria grown in each medium. G, H. Invasion of HeLa cells by SL1344wt bacteria grown in LB (G) or saccharate M9 (H). A one-way ANOVA with Dunnett’s multiple comparisons test was used for peak promoter activity, MFI, and invasion assay data. Data shown are mean ±SD of n = 3 (* p < 0.05).