Fig 5. trkA inactivation restores ΔholC and ΔholC ΔholD viability.

Isolated colonies obtained on M9 as described in the legend of Fig 2 were suspended in MK0 salts and 5 μl drops of serial dilutions were plated on the indicated medium (see S4 Fig for growth on MK1, MK22 and MK115). LB plates were incubated overnight at 37°C and 42°C, and for two days at 30°C. M9 casamino acids plates were incubated for two days at all temperatures. The strains used to generate plasmid-less colonies are: holC, JJC6748; holC ΔtrkA⁸⁴, JJC6827; holC holD, JJC6774; holC holD ΔtrkA⁸⁴, JJC6828.