Skip to main content
. 2016 Jun 9;11(6):e0156468. doi: 10.1371/journal.pone.0156468

Fig 5. PTZ-induced kindling in rats.

Fig 5

(a) Mean number of PTZ-injections needed to produce the first generalized seizure in the three series of PTZ-kindling (see Fig 4). Left part: impact of Tanshinone IIA SO3Na (TIIAS) and Brilliant Blue G (BBG); Middle: impact of AFC-5128 (AFC); Right part: impact of JNJ-47965567 (JNJ) in comparison with the corresponding vehicle controls (n = 12). Animals, which showed no generalized seizure (< stage 3) up to the 20th PTZ-injection, were calculated with “20”. Significance level: *P <0.05, ***P < 0.001 (Mann-Whitney rank sum test). (b) Effects of tanshinone IIA-SO3Na (TIIAS), Brilliant Blue G (BBG), AFC-5128 (AFC) and JNJ-47965567 (JNJ) on PTZ-induced seizures in fully PTZ-kindled rats in comparison with carbamazepine (CBZ) and Ca-valproate (VP). Each pair of columns represents the mean seizure stage (+ SEM) of a tested group, after vehicle treatment (vehicle seizure stage) and after compound treatment (compound seizure stage), indicated as white and coloured column, respectively). The number of animals within the group is shown on the top of the columns, the doses of compounds and the time of pre-medication before PTZ-injection are shown below the columns. Significance level: *P < 0.05, **P < 0.01, ***P < 0.001 (paired t-test).