Fig 6. Graded Smo activity generated by phospho-mimetic mutations in PKA/CK1, Gprk2 and Gish phosphorylation sites.

(A–D") Wing discs expressing the indicated Smo constructs with C765 were immunostained to show the expression of GFP, ptc-lacZ, and En. C765>SmoSDall-CFP induced ectopic en expression in a broader domain of A-compartment cells than C765>SmoSDGPSD-CFP (indicated by the brackets in C", D"). (E) ptc-luciferase reporter assay in Cl8 cells expressing the indicated Smo constructs and treated with Hh-conditioned or control medium. Data are means ± SD of normalized ptc-luc activity from three independent experiments. P-values are Student's t tests. (F) FRET efficiency from SmoSD123-CFP^C/SmoSD123-YFP^C and SmoSDCL-IISA-CFP^C/ SmoSDCL-IISA-YFP^C expressed in S2 cells. Data are means ± SD, n = 10 cells. P-values are Student's t tests.