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. Author manuscript; available in PMC: 2016 Nov 20.
Published in final edited form as: ACS Chem Biol. 2015 Sep 22;10(11):2512–2519. doi: 10.1021/acschembio.5b00711

Figure 2.

Figure 2

BDF2 based α-galactosidase reporter for evaluating A to I RNA editing activity in S. cerevisiae. A) Schematic of reporter mRNA. B) BDF2-derived sequence used as substrate with edited A colored red. Green color indicates sequences changes to maintain open reading frame. C) Full sequence of reporter mRNA near editing site with the encoded protein sequence shown. D) Yeast colony color with BDF2 reporter expressed in the presence of wild type hADAR1-D or the inactive mutant E912A (above) and sequencing of RT-PCR products from reporter mRNA in these samples (bottom). E) Colored phenotype is only apparent using the new BDF2-based reporter and hADAR1-D.