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A) Site-saturation mutagenesis was carried out at hADAR1-D E1008 codon, and the resulting library was screened for the generation of differentially colored yeast colonies. The randomized codon is designed as NNS where N is any natural nucleotide and S is either guanosine or cytosine. B) The phenotypes of yeast co-expressing (1) hADAR1-D E1008Q and GluR B reporter plasmid; (2) hADAR1-D wild type (WT) and GluR B reporter plasmid; and (3) hADAR1-D E1008H and GluR B reporter plasmid.
