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A) Predicted RNA secondary structure surrounding the BDF2 recoding site. The base opposite the editing site is mutated to a guanine in this RNA. B) Comparison of observed rate constants (kobs) for deamination of the BDF2 A•G mismatch substrate RNA by 35 nM hADAR1-D wild type (WT), E1008Q (Q), E1008H (H), and E1008R (R). Error bars indicate SD; n≥3. * indicates no product observed over 1 h reaction time.
