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. 2016 Jun 9;44(3):790–795. doi: 10.1042/BST20160025

Figure 2. Arrangement of an apolipoprotein domain fusion tag surrounding the integral membrane protein EmrE.

Figure 2

Structural reconstruction based on SAXS analysis of ΔspMBP–EmrE–ApoAI* fusion protein [27]. (a) ΔspMBP has been removed from the reconstruction to allow visualization of ApoAI* (orange/yellow) shielding EmrE. (b–c) Side views of the chimaera with ΔspMBP present. Figure reproduced from [27]: Mizrachi, D., Chen, Y., Liu, J., Peng, H., Ke, A., Pollack, L., Turner, R., Auchus, R. and DeLisa, M. (2015) Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy. Nat. Commun. 6.