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. Author manuscript; available in PMC: 2017 May 19.
Published in final edited form as: Cell. 2016 Apr 22;165(5):1238–1254. doi: 10.1016/j.cell.2016.04.032

Figure 6. Generation of Midbrain and Hypothalamic Organoids.

Figure 6

(A–E) Midbrain organoids from human iPSCs. Shown in (A) is a schematic diagram of the midbrain organoid protocol. Shown in (B) are sample images of day 18 organoids (Scale bars: 100 μm) and quantifications. Values represent mean ± SEM (n = 4 organoids each; *P < 0.05, Student’s t-test). Also shown are sample images of immunostaining of midbrain organoids at day 38 (C) and day 75 (D), and monolayer cultures 5 days after dissociation and plating of day 65 midbrain organoids (E). Scale bars: 50 μm.

(F–H) Hypothalamic organoids. Shown in (F) is a schematic diagram of the hypothalamic organoid protocol. Shown are sample images of day 8 (G) and day 40 (H) organoids. Scale bars: 100 μm. Also shown in (H) is a summary of quantification for peptidergic neuronal markers expression in day 40 hypothalamic organoids from 2 iPSC lines. Values represent mean ± SEM (n = 3 organoids each).

Also see Figure S6.