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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Neurochem Int. 2016 May 7;97:49–56. doi: 10.1016/j.neuint.2016.05.004

Fig. 4. Effects of Ashwagandha (A), Sutherlandia (B), and Açaí (C) extracts on LPS-induced ARE-dependent luciferase activity in DI TNC1 astrocytes.

Fig. 4

DI TNC1 astrocytes stably transfected with a luciferase reporter gene driven by the Nrf2/ARE were treated with the indicated concentration of botanical extracts for 1 h followed by LPS (100 ng/mL) or vehicle for 6 h. Luciferase activity was determined, as described in Methods. Results are expressed as the mean ± SEM (n=3) and analyzed by two-way ANOVA with Bonferroni post-tests, where “a” denotes significant differences between LPS+botanical extracts vs. extracts alone (p<0.05 for Sutherlandia; p<0.01 for Ashwagandha; p<0.001 for Açaí); “b” denotes significant differences between LPS+botanical extracts vs. LPS alone (p<0.001); “c” denotes significant differences as compared to 0 μg/mL extract (p<0.001 for each comparison except for Açaí at 6.25 μg/mL, where p<0.01).