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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Cell Host Microbe. 2016 May 19;19(6):882–890. doi: 10.1016/j.chom.2016.05.009

Figure 2. ZIKV NS5 proteins antagonize Type I IFN signaling by targeting STAT2.

Figure 2

(A) ISG54 reporter assay performed in triplicate in 293T cells transfected with increasing amounts of NS5 expressing plasmids. (B) HA-tag IP assay and immunoblot of 293T cells transfected with indicated HA-tagged plasmids. (C) Immunoblot of 293T cells transfected with increasing amounts of DENV2, ZIKV UG and FP NS5 plasmids. (D) Vero cell IFA for colocalization of overexpressed human STAT2-FLAG and ZIKV NS5-HA tagged proteins. (E) Vero cell IFA of endogenous STAT2 levels post IFN treatment for 30 minutes. Error bars represent mean ±SD. Results are representative of three or more independent experiments.