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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Cell Host Microbe. 2016 May 19;19(6):882–890. doi: 10.1016/j.chom.2016.05.009

Figure 3. ZIKV degrades STAT2 in a proteasome dependent manner.

Figure 3

(A) Vero cell IFA of endogenous STAT2 upon ZIKV UG infection and IFN treatment for 30 minutes. (B) qRT-PCR analysis of ISGs and ZIKV UG RNA upon infection in Vero cells for 24h at indicated MOI, following mock treatment or treatment with IFN for 14h. nd=not detected, error bars represent mean ±SD of triplicates. (C) Immunoblot of Vero cells infected with ZIKV UG or ZIKV PR for 24h at indicated MOI. (D) Immunoblot of 293T cells infected with DENV2 and ZIKV UG followed by mock, MG132 (MG) or Lactacystin (LC) proteasome inhibitor treatment. (E) Immunoblot of DENV2 and ZIKV UG infected WT 293T or Ubr4 deficient 293T cells, 24h post infection at MOI 5. (F) HA-tag IP assay and immunoblot of 293T cells transfected with indicated HA-tagged plasmids. Results are representative of three or more independent experiments.