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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Cell Host Microbe. 2016 May 26;19(6):865–873. doi: 10.1016/j.chom.2016.05.003

Figure 3. Antifungal treatment alters the composition of commensal fungal populations of the gut.

Figure 3

(A) Quantitative PCR for fungal 18S rDNA in feces of mice before and after 3 weeks treatment with fluconazole. (B) Bar graphs show relative abundance of specific fungal genera sequences before and after treatment with fluconazole assessed by ITS1 amplicon sequencing. (C) PCR quantification of Candida and Aspergillus DNA, relative to total 18S fungal rDNA, in the feces of mice before and after fluconazole treatment. (D) PCoA analysis of fungal communities in control mice and mice treated with fluconazole or amphotericin. (E) Taxonomic distribution of most abundant fungal species in control mice and mice treated with fluconazole or amphotericin. LEfSe analysis on samples from control mice and mice treated with fluconazole or amphotericin showed significant decrease (F) of some or expansion (G) of other fungal species. The horizontal straight lines in the panels (F, G) indicate the group means, and the dotted lines indicate the group medians. Each bar represents an individual mouse. Data are representative of three independent sequencing experiments with 5 mice/group for each condition. See also Figure S2.