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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Oral Dis. 2016 Mar 10;22(5):391–398. doi: 10.1111/odi.12456

Figure 1. Chromatographic separation of miconazole dissolved in saliva or PBS.

Figure 1

Miconazole, dissolved in 10% human saliva or PBS, was isolated on a C-18 HPLC column as described in the Methods section. Panel A: Chromatogram of 0.2 µg/mL miconazole dissolved in 10% saliva. Miconazole elutes at 6.7 mins and the area under the curve (proportional to concentration) is shown in the box and calculated by the HPLC. Panel B: Chromatogram of 0.2 µg/ml miconazole dissolved in PBS. Miconazole elutes in an equivalent manner as found in Panel A. Panel C: A representative standard curve for miconazole dissolved in 10% saliva. Each point represents the mean±SEM for 5 determinations. Irrespective of the solvent used, dose-dependency and linearity were equivalent to that shown.