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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Oral Dis. 2016 Mar 10;22(5):391–398. doi: 10.1111/odi.12456

Figure 4. Adsorption of salivary proteins to PMMA-g-PNVP disks.

Figure 4

Panel A: PMMA-g-PNVP disks immersed in 10% human saliva for 20 days were stained with FITC and viewed in a confocal microscope. Positive staining was observed on disks incubated in saliva (left image) but not PBS (right image). Panel B: Quantitation of protein adsorption using the microBCA assay. Disks were incubated for 20 days in 10%, 50%, and 100% saliva, the adsorbed proteins released with SDS, and then assayed. Protein adsorption significantly increased with increasing saliva concentration. *P ≤ 0.015 versus 10%, ** P ≤ 0.001 versus 10%.