Table 2.
Summary of SARS-CoV receptor, NP expression, and genomic RNA in the immune cells of infected macaques
| Immune cell | ACE2 staining | Upper respiratory tract | Tonsils and MALT | Lung | Draining LNs | ||||
|---|---|---|---|---|---|---|---|---|---|
| NP | RNA | NP | RNA | NP | RNA | NP | RNA | ||
| MAC387+ | +++ | − | − | − | − | − | − | ND | − |
| CD163+ | + | ++ | − | + | − | + | + | ND | + |
| CD68+ | +++ | ++ | − | + | + | + | + | ND | + |
| Langerin+ | ++ | ++ | − | +++ | − | + | − | ND | − |
| DC-SIGN+ | ND | NA | NA | − | − | NA | NA | ND | + |
| CD3+ | − | − | − | − | − | − | − | ND | − |
| CD20+ | − | − | − | − | − | − | − | ND | − |
Abbreviations: ACE2, angiotensin-converting enzyme 2; DC-SIGN, dendritic cell–specific intercellular adhesion molecule-3-grabbing non-integrin; LN, lymph node; MALT, mucosa-associated lymph tissue; NA, no cells detected in the tested tissue; ND, not done; NP, nucleoprotein; SARS-CoV, severe acute respiratory syndrome coronavirus.The results of staining for SARS-CoV, NP, and RNA were quantified as follows: undetectable positive signals=−; 1–2 positive cells per 10 cells=+; 3–7 positive cells per 10 cells=++; >7 positive cells per 10 cells=+++.