Fig. 2.

Isolation of Zika virus from semen sample. (A) Temporal change of cycle threshold (Ct) value for Zika virus RT-PCR in the culture of first passage. (B) Transmission electron microscopy image of Vero cells infected with Zika virus. White arrows denote virus particles. Black scale bar indicates 200 nm. (C, D) Immunofluorescence assay shows that Zika virus-infected Vero cells reacted with human convalescent anti-Zika virus IgG-positive serum (C) and did not with control serum (D). White scale bar denotes 100 μm.