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. 2016 Jun 10;7:217. doi: 10.3389/fimmu.2016.00217

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Copyright © 2016 Chatzipanagiotou, Ioannidis, Trikka-Graphakos, Charalampaki, Sereti, Piccinini, Higgins, Buranda, Durvasula, Hoogesteijn, Tegos and Rivas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of subsets detected by structure III. The immune profiles of subsets detected in Figures 8A,B were investigated. Discrimination was repeatable, even when a different data structure was utilized. While patterns differed slightly [study I (n = 36) showed four subsets, and one pattern (“vertical”) was only composed of one outcome (non-survivors) (A)], study II (n = 69) detected three subsets, with two outcomes per subset (B). The “left” and “right” subsets of both studies reproduced the information observed in Figure 8: while the “left” subset did not show within-subset differences, the lymphocyte and neutrophil percentage intervals of the “right” subset did not overlap. The “vertical” subset of study II seemed to correspond to both the “vertical” and “lower left” subsets of study I. Horizontal lines show some data subsets that did not overlap.