Table 2.
Output of Volcano analysis: metabolites resulting differential in lettuce root exposed to zinc stress against control, grouped according to functional classes.
| Metabolites | p (Corr) | FC (abs) | Regulation | |
|---|---|---|---|---|
| Carbohydrates | 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate | 7.12E-15 | 138,963 | Down |
| 1-kestotriosea | 2.37E-15 | 13,856,115 | Down | |
| 1,1-kestotetraosea | 8.18E-08 | 18,210,966 | Down | |
| Maltoheptaosea | 6.66E-23 | 23,748,209 | Down | |
| β-D-apiofuranosyl-(1-6)-D-glucose | 4.54E-09 | 10,028,662 | Down | |
| D-hexose –phosphatea | 0 | 16 | Down | |
| Lipids | Digitoxigenin 3-O-beta-D-quinovoside | 7.60E-09 | 11,217,574 | Down |
| Eicosapentaenoyl-CoA | 6.47E-27 | 21,449,984 | Down | |
| Solasodine 3-O-beta-D-glucopyranoside | 1.38E-09 | 8,561,974 | Down | |
| Hexadecanedioyl-CoA | 3.20E-23 | 27,931,486 | Down | |
| CDP-1-18:1(9Z)-2-16:0-glycerola | 8.77E-07 | 70,597,266 | Down | |
| Dihomo gamma-linolenoyl-2-enoyl-CoA | 0 | 16 | Up | |
| Phosphoryl-ethanolaminea | 0 | 16 | Up | |
| Acetoacetate | 0 | 16 | Up | |
| CDP-1-18:1(9Z)-2-18:1(9Z)-glycerola | 1.05E-06 | 7,139,225 | Down | |
| Phenolics | 8C-glucosyl-2,5,7-trihydroxyflavanone | 5.55E-08 | 9,611,869 | Down |
| Isovitexin-7-O-xyloside | 8.55E-15 | 11,610,447 | Down | |
| Afrormosin-7-O-glucoside-6″-O-malonate | 1.28E-14 | 12,522,652 | Down | |
| Pelargonidin 3,7-di-O-beta;-D-glucoside | 0 | 16 | Up | |
| Apigenin-7-O-gentiobioside | 0 | 16 | Down | |
| Catechinb | 0 | 16 | Up | |
| Oxidative stress | 4,9,13-trimethyltetradeca-2,4,6,8,10,12-hexaene-1,14-dial | 0 | 16 | Up |
| 4-trans-3-oxo-undecenoyl-CoA | 9.86E-29 | 23,782,549 | Down | |
| Hormones | Gibberellin A34-catabolitea | 2.27E-11 | 7,507,979 | Down |
| Trans-zeatinribosidediphosphatea | 0 | 16 | Down | |
| Glucosinolates | 4-sinapoyloxybutylglucosinolate | 7.12E-15 | 57,585,897 | Down |
| Benzyl-desulfoglucosinolate | 1.24E-04 | 11,503,575 | Up | |
| (E)-indol-3-ylacetaldoxime | 1.53E-17 | 25,664,116 | Down | |
| Indole-3-acetohydroximoyl-cysteinylglycine | 0 | 16 | Up | |
| Indolepropanolphosphate | 0 | 24,768,307 | Up | |
| Phytoalexins | Zealexin B1a | 1.72E-07 | 700,998 | Up |
| Alkaloids | Cyclo-dopaglucuronylglucoside | 4.54E-05 | 43,532,617 | Up |
| Gamma-coniceine | 8.00E-30 | 13,491,078 | Up | |
| S-canadine | 3.27E-04 | 27,555,862 | Down | |
| Others | L-carnitine | 4.95E-04 | 863,446 | Up |
| Pyridoxamine | 1.38E-13 | 18,502,228 | Down | |
| D-myo-inositol (1,2,3,4,6)-pentaikisphosphatea | 0 | 16 | Down | |
| Alpha-ethyl-L-glutamatea | 3.28E-09 | 17,081,303 | Down | |
| 2′-deoxyadenosine | 4.74E-04 | 11,663,641 | Down | |
| 4-acetamidobutanal | 3.72E-04 | 8,858,312 | Up | |
| D-xylonolactone | 0 | 22,147,355 | Up | |
| Dihydroxyferuoyl-sinapoyl-spermidinea | 0 | 16 | Up | |
| Spermidineb | 0 | 16 | Up | |
| 5′-Methylthioadenosineb | 1.75E+02 | 10,369,577 | Down | |
| S-Adenosyl-L-homocysteineb | 7.63E+03 | 16 | Up | |
| Pheophorbide ab | 5.28E-03 | 17,494,915 | Up |
For each compound, unpaired t-test p-values (Bonferroni multiple testing correction), absolute fold-change (FC) and regulation are provided. According to Mass Profiler Professional output, values of p (Corr) = 0 or FC = 16 denote very high significance at t-test or extremely high fold-change, respectively.
a
Confirmed by partial least squares discriminant analysis (PLS-DA).
b
Resulted from KEGG database using the Pathway-to-PCDL tool.