Table 2.
Primers used to test for the presence of virulence genes.
Target gene∗ | Primers | Fragment size (pb) |
---|---|---|
Agg | AAGAAAAAGAAGTAGACCAAC AAACGGCAAGACAAGTAAATA | 1,553 |
GelE | ACCCCGTATCATTGGTTT ACGCATTGCTTTTCCATC | 419 |
esp | TTGCTAATGCTACTCCACGACC GCGTCAACACTTGCATTGCCGAA | 933 |
efaAfs | GACAGACCCTCACGAATA AGTTCATCATGCTGTAGTA | 705 |
efaAfm | AACAGATCCGCATGAATA CATTTCATCATCTGATAGTA | 735 |
cpd | TGGTGGGTTATTTTTCAATTC TACGGCTCTGGCTTACTA | 782 |
Cob | AACATTCAGCAAACAAAGC TTGTCATAAAGAGTGGTCAT | 1,405 |
Ccf | GGGAATTGAGTAGTGAAGAAG AGCCGCTAAAATCGGTAAAAT | 543 |
cylLL | GATGGAGGGTAAGAATTATGG GCTTCACCTCACTAAGTTTTATAG | 253 |
cylLS | GAAGCACAGTGCTAAATAAGG GTATAAGAGGGCTAGTTTCAC | 240 |
cylM | AAAAGGAGTGCTTACATGGAAGAT CATAACCCACACCACTGATTCC | 2,940 |
cylB | AAGTACACTAGTACAACTAAGGGA ACAGTGAACGATATAACTCGCTATT | 2,020 |
CylA | TAGCGAGTTATATCGTTCACTGTA CTCACCTCTTTGTATTTAAGCATG | 1,282 |
∗Agg (Aggregation protein), gelE (gelatinase), esp (cell-wall-associated protein) efaAfm and efaAfs (cell wall adhesins), cpd, cob and ccf (sex pheromones, chemotactic for human leukocytes, facilitate conjugation), cylLL and cylLS (Cytolysin precursor), cylM (post-translational modification of cytolysin), cylB (transport of cytolysin) and cylA (activation of cytolysin).