Table 3.
Biosurfactant production by the LAB strains.
| Method | |||
|---|---|---|---|
| Strains | Hemolysis in blood agara | Drop collapse testb | MATH testc (%) |
| Lactobacillus sakei MBSa1 bac+ | ++ | ++ | 88.9 ± 0.14 |
| Lactobacillus curvatus MBSa3 bac+ | ++ | ++ | 76.4 ± 0.23 |
| Lactococcus lactis VB69 bac+ | + | ++ | 95.2 ± 0.09 |
| Lactococcus lactis VB94 bac+ | – | + | 95.1 ± 0.13 |
| Lactobacillus casei 40 bac– | + | + | 77.2 ± 0.30 |
| Lactobacillus helveticus 352 bac– | + | + | 91.2 ± 0.4 |
| Lactococcus lactis 368 bac– | ++ | ++ | 81.2 ± 0.11 |
| Weissella virisdescens 113 bac– | ++ | ++ | 93.9 ± 0.32 |
a(+) incomplete hemolysis; (++) complete hemolysis with a diameter of lysis < 1 cm; (+++) complete hemolysis with a diameter of lysis > 1 cm but < 3 cm; and (++++) complete hemolysis with a diameter of lysis > 3 cm and green colonies.
bFlat drops with scoring system ranging from + to ++++ corresponding to partial to complete spreading on the oil Surface. Rounded drops were scored as negative—indicative of the lack of biosurfactant production.
cPercent of bacterial cell surface hydrophobicity.