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. 2016 Jun 10;7:863. doi: 10.3389/fmicb.2016.00863

Table 3.

Biosurfactant production by the LAB strains.

Method
Strains Hemolysis in blood agara Drop collapse testb MATH testc (%)
Lactobacillus sakei MBSa1 bac+ ++ ++ 88.9 ± 0.14
Lactobacillus curvatus MBSa3 bac+ ++ ++ 76.4 ± 0.23
Lactococcus lactis VB69 bac+ + ++ 95.2 ± 0.09
Lactococcus lactis VB94 bac+ + 95.1 ± 0.13
Lactobacillus casei 40 bac– + + 77.2 ± 0.30
Lactobacillus helveticus 352 bac– + + 91.2 ± 0.4
Lactococcus lactis 368 bac– ++ ++ 81.2 ± 0.11
Weissella virisdescens 113 bac– ++ ++ 93.9 ± 0.32

a(+) incomplete hemolysis; (++) complete hemolysis with a diameter of lysis < 1 cm; (+++) complete hemolysis with a diameter of lysis > 1 cm but < 3 cm; and (++++) complete hemolysis with a diameter of lysis > 3 cm and green colonies.

bFlat drops with scoring system ranging from + to ++++ corresponding to partial to complete spreading on the oil Surface. Rounded drops were scored as negative—indicative of the lack of biosurfactant production.

cPercent of bacterial cell surface hydrophobicity.

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