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. 2016 Jun 10;9:43. doi: 10.3389/fnmol.2016.00043

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Copyright © 2016 Pinto, Pedro, Costa and Almeida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enhanced UiFC fluorescence at newly formed presynaptic terminals. (A) UiFC signal on bead-induced presynaptic clusters. Neurons were co-transfected with UiFC (green) and VGluT1mCherry (red) and poly-D-lysine (PDL)-coated beads added for a total of 8 h. UiFC signal was greatly enhanced in bead-induced presynaptic clusters. Scale bar represents 50 μm. (B) Enlarged images of box in (A). Scale bar represents 5 μm. (C) Quantitative values of VGluT1mCherry and UiFC intensity at on- and off-bead sites (all beads included in the analysis). Results are normalized to off-bead and shown as mean ± SEM. Statistical analysis by Wilcoxon paired t-test (***p < 0.001 between on and off for each marker). (D) Percentage of beads inducing accumulation of UiFC, VGluT1mCherry and both on contacting axons in relation to adjacent off-sites. A total of 152 beads were analyzed from two independent experiments.