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. 2016 Jun;186(6):1427–1434. doi: 10.1016/j.ajpath.2016.01.020

Figure 1.

Figure 1

Relationship of 5-hydroxymethylcytosine (5-hmC) and nestin reactivity in primary and metastatic human melanomas. A: Line diagrams represent relative biomarker scores; the majority cases show patterns of higher nestin and lower 5-hmC. Three cases show inversion of these patterns (dashed boxes). B: Representative IHC staining for 5-hmC and nestin in primary and metastatic human melanomas; the patients in A and C show the dominant pattern of disparity between high nestin and low 5-hmC, and the patients in B and D represent the reciprocal pattern. C: Representative immunofluorescence staining demonstrating the trend for higher nestin and lower 5-hmC levels in primary and metastatic human melanomas. Red indicates nuclear 5-hmC; green, cytoplasmic nestin in green; blue nuclear stain, DAPI. D: Spearman correlation of the immunoreactivity of 5-hmC and nestin expression. P = 0.037; ρ = −0.411 in primary melanoma cases (D). Original magnification, ×40. H&E, hematoxylin and eosin.