Skip to main content
. 2016 Jun;186(6):1427–1434. doi: 10.1016/j.ajpath.2016.01.020

Figure 2.

Figure 2

Effects of ten-eleven translocation methylcytosine dioxygenase (TET2)-mediated 5-hydroxymethylcytosine (5-hmC) on nestin gene and protein expression in cultured melanoma cells (A2058 and Meljuso). A: Schematic diagram of melanoma cell line transfected with full-length TET2 or enzymatically inactive TET2 mutant control. B: Up-regulation of 5-hmC by full-length TET2 in A2058 cells but not by mutant TET2 control was confirmed by immune dot blot. The methylene blue staining was used as a total genomic DNA loading control. RT-PCR shows a significant decrease in nestin mRNA expression as a result of TET2 overexpression. C: Significant decrease in nestin gene expression is also observed in Meljuso TET2 overexpression (OE) cell lines compared to the control cells by RT-PCR. Data are expressed as means ± SEM. n = 3 (B and C). ∗P < 0.05, ∗∗P < 0.01.