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. 2016 Jun;186(6):1688–1700. doi: 10.1016/j.ajpath.2016.01.023

Figure 5.

Figure 5

Angiotensin-converting enzyme (ACE)-2 overexpression prevents diabetes-induced increase of macrophage/microglial cells. A: Prevention cohort; representative immunofluorescence images of F4/80-positive (red arrowheads) and F4/80–green fluorescent protein (GFP)–positive (yellow arrowheads) cells in GFP-chimeric, streptozotocin (STZ)–diabetic, and vehicle (Veh)–control (Con) mouse retinas. B: Prevention cohort; quantification of F4/80-positive cells per square millimeter. C: Prevention cohort; quantification of F4/80-GFP–positive cells per square millimeter. D: Reversal cohort; representative immunofluorescence images of F4/80-positive (red arrowheads) and F4/80-GFP–positive (yellow arrowheads) cells in GFP-chimeric, STZ-diabetic, and Veh-Con mouse retinas. E: Reversal cohort; quantification of F4/80-positive cells per square millimeter. F: Reversal cohort; quantification of F4/80-GFP–positive cells per square millimeter. Data are expressed as means ± SEM. ∗P < 0.05 versus STZ-Con. Scale bar = 100 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer.