Figure 5.

IBMPFD-relevant VCP mutants do not delay stress granule resolution after heat shock treatment in C2C12 cells. A: Cells expressing VCP(R155H) or VCP(A232E) show similar TiA-1⁺ stress granules compared with wt VCP-expressing cells on all conditions: no treatment, immediately after heat shock, or 30 minutes after heat shock (right). B: Representative images of the heat shock and recovery condition. The dsRED tag was fused to the different VCP constructs to allow identification of transfected cells. Arrowheads indicate stress granules. n = 100 cells per well (7 to 8 wells per condition). Scale bar = 50 μm. IBMPFD, inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia; TiA-1, T-intracellular antigen-1; VCP, valosin-containing protein; wt, wild-type.