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. 2016 Jun;186(6):1466–1480. doi: 10.1016/j.ajpath.2016.01.019

Figure 7.

Figure 7

Exogenous IL-1 receptor antagonist (IL-1Ra) partially rescues suppressed expression of CXCL10, CXCL5, and chemokine ligand (CCL) 5 in healing diabetes mellitus (DM) corneas. A: Protein array analysis reveals the effect of exogenous IL-1Ra on cytokine/chemokine expression. Selected images for CXCL10, CXCL5, and CCL5 with Western blot analysis of actin as the loading control are shown. B: Real-time quantitative PCR analysis of Cxcl10, Cxcl5, and Ccl5 in CECs. C: Enzyme-linked immunosorbent assay of CXCL10 in corneal epithelial cells. D: Immunofluorescence staining of CXCL10 in corneal frozen sections (6 μm thick). Arrows indicate epithelial leading edge. Two independent experiments were performed. Data are given as means ± SEM (B and C). n = 3 (B and C). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (one-way analysis of variance). BSA, bovine serum albumin; NL, normal.