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. Author manuscript; available in PMC: 2016 Jul 6.
Published in final edited form as: Am J Respir Crit Care Med. 2011 Jun 16;184(6):724–731. doi: 10.1164/rccm.201012-2033OC

Figure 5. RANTES/CCL5 neutralization attenuates IH-induced aorta remodeling.

Figure 5

Mice were exposed to intermittent hypoxia (IH) or air (N) for 14 days, and treated either with the anti-RANTES/CCL5 monoclonal antibody or with the control IgG throughout the exposure. (A) Intima-media thickness (n=7–8 each). (B) Cytosolic α-smooth muscle actin (α-SMA) expression with representative immunoblotting and quantitative analysis (n=5–7 each). (C) Nuclear NFkB-p50 expression with representative immunoblotting and quantitative analysis (n=4–7 each). (D) Quantification of T-cell infiltration in the aortic wall (n=7–8 each). (E) Representative RANTES/CCL5 immunostaining (10x20 magnification). (F) IFNγ mRNA expression normalized to ubiquitin (n=4–9 each). *p<0.05 vs N-IgG; †p<0.05 vs anti-RANTES treated IH-mice. (G) Regulation pathway of NFkB activation and subsequent leukocyte recruitment and activation (adapted from Ye et al, (44)).