Fig. 3.

Immuno-EM analyses of Huh7.5 cells on day 6 post infection with HCVcc, prepared by freeze substitution. a, b Immunogold labeling with a monoclonal antibody against core protein was observed over the entire surface of lipid droplets (LD) and on the membrane of the adjacent contiguous vesicles (CV). c, d Immunogold labeling with a monoclonal antibody against NS5A was more discrete than that for core protein, but was also observed in some areas of the LD surface (arrows in c), and on the membrane of the adjacent CVs (arrows in d). e Immunogold labeling with the monoclonal antibody against NS5A was also observed on double-membrane vesicle (DMV) membranes (arrows). f Immunogold labeling with the monoclonal antibody against dsRNA was observed within the DMVs or associated with DMV membranes (arrows). These observations were specific to Huh7.5-infected cells, as no immunogold labeling was detected in the uninfected Huh7.5 cells (not shown)