Figure 4. CE challenge increases and CD1d blockage decreases NKT cells in BALF and spleen of mice.
(a) Immunization protocol (b,c) NKT cells in BALF and spleen. NKT cells are expressed as the percentage NKT cells, i.e. % proportion of the NKT cells out of the total cells in BALF or spleen. In part b, vehicle control: PBS; mice sensitized and challenged with cockroach extract and pre-administered with PBS: CE-CE-PBS; with isotype: CE-CE-Isotype; or with anti-CD1d monoclonal antibody (mAb): CE-CE-mAb CD1d. In part c, vehicle control: PBS; Mice sensitized with cockroach extract and exposed to LPC and pre-administered with PBS: CE-LPC-PBS; or with isotype: CE-LPC-Isotype; or with anti-CD1d mAb: CE-LPC-mAB CD1d. Data represent the means ± SEM of values from 4 mice. *p < 0.05 versus PBS; #p < 0.05 versus CE-CE-PBS, $p < 0.05 versus CE-LPC-PBS.