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. 2016 Jun 10;6:26351. doi: 10.1038/srep26351

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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(A) A multivariate Cox analysis of the clinical and pathological factors showed predictive value with respect to OS in gallbladder cancer patients. Generally, PKM2 and pN stage showed prognostic value for predicting OS. Specifically, PKM2 demonstrated prominent prognostic value in patients with early stage gallbladder cancer (i.e., TNM stages I and II), and the combination of the TNM stages and the PKM2 staining demonstrated better predictive value than either alone. (B) A multivariate Cox analysis of the clinical and pathological factors demonstrated predictive value with respect to DFS in gallbladder cancer patients. Generally, PKM2 and pN stage showed prognostic value for predicting DFS. Specifically, PKM2 exhibited better prognostic value in patients with early stage gallbladder cancer (i.e., TNM stages I and II). The combination of TNM stage and PKM2 staining showed better predictive value than either alone. (C–F) A rank-sum test compared PKM2 expression levels and the combined risk factors to predict OS and DFS.