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. 2016 Jun 10;6:27904. doi: 10.1038/srep27904

Figure 2. miR-20a-5p and miR-20b-5p promote myoblast differentiation.

Figure 2

(a) The relative mRNA expression of MYOD, MYOG, MyHC and Myomaker after transfection of miR-20a-5p, miR-20b-5p or NC mimic. (b) MyHC staining of myoblasts at 72 h after transfection of miR-20a-5p, miR-20b-5p or NC mimic. (c) Number of myotubes at 72 h after transfection of miR-20a-5p, miR-20b-5p or NC mimic. (d) Myotube area (%) at 72 h after transfection of miR-20a-5p, miR-20b-5p or NC mimic. (e) The relative mRNA expression of MYOD, MYOG, MyHC and Myomaker after transfection of miR-20a-5p inhibitor, miR-20b-5p inhibitor or NC inhibitor. (f) The relative mRNA expression of MYOD, MYOG, MyHC and Myomaker after transfection of miR-20a-5p/miR-20b-5p mixed inhibitors or NC inhibitor. (g) MyHC staining of myoblasts at 72 h after transfection of miR-20a-5p/miR-20b-5p mixed inhibitors or NC inhibitor. (h) Number of myotubes at 72 h after transfection of miR-20a-5p/miR-20b-5p mixed inhibitors or NC inhibitor. (i) Myotube area (%) at 72 h after transfection of miR-20a-5p/miR-20b-5p mixed inhibitor or NC inhibitor. Results are shown as the mean ± sem of three independent experiments. One sample t test was used to analysis the statistical differences between groups. *p < 0.05; **p < 0.01.