Skip to main content
. Author manuscript; available in PMC: 2017 Jun 6.
Published in final edited form as: Dev Cell. 2016 Jun 6;37(5):444–457. doi: 10.1016/j.devcel.2016.05.004

Figure1. Lack of p53-dependent apoptosis in papillar precursors.

Figure1

A,B.3rd instar larval wing imaginal discs. A.Nuclei in undamaged disc. B.Pycnotic nuclei (arrows) in irradiated disc, 6 hrs. post IR. C,D.2nd instar rectum (during papillar precursor endocycling). C.Nuclei in unirradiated rectum. D.No pycnotic nuclei in rectum, 6 hrs. post IR. E.% pycnotic nuclei from A-D (wing data-blue, rectum data-red). Numbers indicate mean % pycnotic nuclei/animal (N=minimum 10 animals/condition, multiple replicates). *= significant to p<.001, two-tailed T-test. Bars=standard deviation. F.UAS-p53 expression in rectum (using byn-Gal4) does not induce pycnotic nuclei. G.UAS hid, UAS rpr co-expression in rectum (Methods) does induce pycnotic nuclei. DAPI=DNA in all images. Scale bar=10μm.