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. 2016 Jun 10;6:27539. doi: 10.1038/srep27539

Figure 2. Curcumin does not modulate the level of Tat mRNA.

Figure 2

(A) Myc-Tat transfected HEK-293T cells were treated with curcumin for 8 hrs and total RNA was isolated using TRIZOL reagent followed by RT-PCR using Tat and GAPDH primers. The gel image is a representative of three independent experiments. (B) Using ImageJ the band intensity of Tat and GAPDH was quantified and plotted as Tat/GAPDH. P value was calculated by a two-tailed t-test (*P < 0.05, **P < 0.01; NS, not significant). (C) Myc-Tat was transfected to HEK-293T cells, 24 hrs post transfection the cell culture medium was applied on fresh HEK-293T cells followed by treatment with curcumin for 6 hrs. Western blotting was done to detect the Tat protein. (D) In a 24 well plate format TZM-bl cells were transfected with 0.2 μg of Myc Tat, after 36 hrs treated with increasing dose of curcumin for 12 hrs and luciferase activity was measured and the mean of three independent experiments was plotted. P value was calculated by a two-tailed t-test (*P < 0.05, **P < 0.01; NS, not significant). (E) Similarly un-transfected TZM-bl cells were also treated with curcumin and luciferase activity was measured and the mean of three independent experiments was plotted. P value was calculated by a two-tailed t-test (*P < 0.05, **P < 0.01; NS, not significant).